Muscle length, shortening, myoplasmic [Ca2+], and activation of arterial smooth muscle.

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Muscle length, shortening, myoplasmic [Ca2+], and activation of arterial smooth muscle.

The effect of muscle length on smooth muscle contraction was evaluated by measuring myoplasmic [Ca2+] (with aequorin), myosin light chain phosphorylation, length, and isometric stress in histamine-stimulated swine carotid media preparations. Tissues were equilibrated at the optimal length for stress development (Lo). Isometric contractions at short tissue lengths (0.7 Lo) were associated with a...

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Myoplasmic [Ca2+] determines myosin phosphorylation in agonist-stimulated swine arterial smooth muscle.

Our objective was to test the hypotheses that 1) myoplasmic [Ca2+] is the primary determinant of crossbridge phosphorylation and that 2) phosphorylation is the primary determinant of crossbridge interactions with the thin filament in swine carotid arterial smooth muscle. We tested these hypotheses by evaluating the relation between aequorin-estimated myoplasmic [Ca2+], myosin light chain phosph...

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Na-Ca Exchange, Myoplasmic Ca Concentration, and Contraction of Arterial Smooth Muscle

Na-Ca exchange is proposed to be an important regulator of myoplasmic intracellular Ca concentration ([Ca]|) and contraction in vascular smooth muscle. We investigated the role of Na-Ca exchange in regulating [Ca], in swine carotid arterial tissues that were loaded with aequorin to allow simultaneous measurement of [Ca], and force. Reversal of Na-Ca exchange, by reduction of extracellular Na co...

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ژورنال

عنوان ژورنال: Circulation Research

سال: 1990

ISSN: 0009-7330,1524-4571

DOI: 10.1161/01.res.66.5.1354